2. Apoptosis Anti-infection
  3. TNF Receptor Bacterial
  4. Adalimumab

Adalimumab  (Synonyms: 阿达木单抗; Anti-Human TNF-alpha, Human Antibody)

目录号: HY-P9908 纯度: 98.28%
COA

摩尔计算器

Adalimumab 是一种人源的单克隆 IgG1 抗体，靶向肿瘤坏死因子α (TNF-α)。

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CAS No. : 331731-18-1

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规格 价格 是否有货 数量
1 mg ￥2700
In-stock
5 mg ￥5700
In-stock
10 mg ￥8835
In-stock
25 mg 询价
50 mg 询价

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Customer Review

Adalimumab 相关抗体

Top Publications Citing Use of Products

查看 TNF Receptor 亚型特异性产品:

查看所有亚型
TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Adalimumab is a human monoclonal IgG1 antibody targeting tumour necrosis factor α (TNF-α).

IC50 & Target

TNF-α[1]
体外研究
(In Vitro)

使用 Adalimumab 处理 HUVEC 长达 12 小时，显示对其细胞活力没有不利影响，即使 Adalimumab 的浓度比本研究中使用的浓度高 10 倍[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Adalimumab 相关抗体:
体内研究
(In Vivo)

Adalimumab 的消除半衰期很长 (t1/2=~20 天)[1]。
荧光标记的 Adalimumab 富集主要在油红 O 阳性病变中观察到，证明药物进入动脉粥样硬化斑块的良好组织渗透性，在溶剂对照注射到高胆固醇血症 Ldlr-/- 老鼠后，血管壁无背景荧光[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

阿达木单抗；阿达莫单抗
运输条件

Shipping with dry ice.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Human IgG1 kappa
Biological Activity
  • Loaded Adalimumab on AHC2 biosensor, can bind TNF-alpha/TNFSF2 Protein, Human (177a.a, His, HY-P700291) with an affinity constant of 2.247E-10 M as determined in BLI assay.
  • Immobilized TNF-alpha/TNFSF2 Protein, Human (HY-P7058) can bind Adalimumab. The EC50 for this effect is 6.753 ng/mL.
纯度 & 产品资料

纯度: 99.62%

COA (264 KB) SDS-PAGE (81 KB) SEC-HPLC (110 KB)

抗体抑制剂使用指南 (1152 KB)
参考文献
Cell Assay
[2]

1×104 HUVECs are plated per well of 96 well plate in EGM. After reaching sub-confluency cells are cultured in different concentration of Adalimumab (0.01-10 μg/mL), IgG isotype control (10 μg/mL) and 0.1% Triton-X as positive control in 5% CO2 at 37°C. After 12 hours, 10% Alamar blue is added to each well and kept in incubator for another 6 hours. Absorbance of oxidized alamar blue is measured at 600 nm on Infinite M200 pro plate reader. All measurements are performed in triplicate[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice[2]
In order to analyze the vascular distribution of Adalimumab, the substance is labelled using the DyLight 549 Microscale Antibody Labeling Kit. Briefly, one vial of DyLight 549 Reagent is dissolved in 1 mg Adalimumab solution (in PBS) and purified with a resin spin column to remove excess fluor. Hypercholesterolemic Ldlr-/- mice are injected with fluorophore-labelled Adalimumab (8.0 mg/kg in 200 μL PBS i.p.) for two consecutive days and sacrificed 12 hours after last injection. Vehicle injection (200 μL PBS) is used as control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Adalimumab 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Adalimumab331731-18-1Anti-Human TNF-alpha, Human Antibody阿达木单抗阿达莫单抗TNF ReceptorBacterialTumor Necrosis Factor ReceptorTNFRInhibitorinhibitorinhibit

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